COST Aseptic Techniques & Infection Control 2

Question 1

Which organism is used in biological indicators to monitor steam sterilization cycles?

Accepted Answer

Geobacillus stearothermophilus

Answer

Geobacillus stearothermophilus spores resist moist heat strongly, so they are the standard challenge organism for steam sterilizers.

Question 2

What is the main purpose of the Bowie-Dick test run in a prevacuum steam sterilizer?

Accepted Answer

To check air removal and steam penetration

Answer

The Bowie-Dick test detects residual air pockets that would block steam penetration in dynamic air-removal sterilizers.

Question 3

A strip of autoclave tape on a wrapped ophthalmic tray has changed color. What does this confirm?

Accepted Answer

The tray was exposed to a sterilization process

Answer

External process indicators only show that the package went through a cycle, not that sterility was achieved.

Question 4

Which sterilization method is best suited to heat- and moisture-sensitive ophthalmic instruments but needs a long aeration period afterward?

Accepted Answer

Ethylene oxide gas

Answer

Ethylene oxide works at low temperatures but leaves toxic residues, so items need prolonged aeration before use.

Question 5

Which packaging material should NOT be used in a hydrogen peroxide gas plasma sterilizer?

Accepted Answer

Cellulose-based paper or linen

Answer

Cellulose absorbs hydrogen peroxide and can cause the cycle to abort or fail.

COST - Certified Ophthalmic Surgical Technologist Practice Test