CLT Clinical Chemistry & Biochemical Analysis 2

Question 1

Which of the following best describes the Beer-Lambert Law as applied to spectrophotometry in clinical chemistry?

Accepted Answer

Absorbance is directly proportional to concentration and path length

Answer

Beer-Lambert Law states that absorbance equals the product of molar absorptivity, path length, and concentration, meaning absorbance increases linearly with concentration.

Question 2

Hemoglobin A1c (HbA1c) reflects average blood glucose control over approximately what time period?

Accepted Answer

8–12 weeks

Answer

HbA1c reflects the average plasma glucose over the preceding 8–12 weeks (approximately 3 months), corresponding to the lifespan of red blood cells.

Question 3

Troponin I or T elevation in a patient with chest pain is most indicative of which condition?

Accepted Answer

Acute myocardial infarction

Answer

Cardiac troponins I and T are released from injured myocardial cells and are the gold-standard biomarkers for acute myocardial infarction.

Question 4

Which method is considered the reference (gold standard) for measuring serum cholesterol?

Accepted Answer

Abell-Kendall method

Answer

The Abell-Kendall method (saponification followed by Liebermann-Burchard colorimetry under controlled conditions) is the CDC reference method for total cholesterol.

Question 5

An arterial blood gas result shows pH 7.28, pCO2 55 mmHg, and HCO3 26 mEq/L. What is the primary acid-base disturbance?

Accepted Answer

Respiratory acidosis

Answer

Elevated pCO2 (>45 mmHg) with a low pH indicates respiratory acidosis caused by CO2 retention.

(CLT) Clinical Laboratory Technologist Certification Practice Test

(CLT) Clinical Laboratory Technologist Certification Practice Test

CLT Clinical Chemistry & Biochemical Analysis 2