CHT Laboratory Management & Safety 2

Question 1

What is the primary purpose of running a negative control in a Luminex SAB HLA antibody assay?

Accepted Answer

To establish baseline non-specific background fluorescence for result interpretation

Answer

Negative controls (normal human serum or buffer) establish the background MFI level; patient MFI values must exceed this background to be considered true positive signals.

Question 2

What action should be taken when a reagent lot number changes for a critical assay in a histocompatibility laboratory?

Accepted Answer

Perform parallel testing with the old and new lot to verify equivalent performance before clinical use

Answer

Lot-to-lot verification ensures that the new reagent lot performs equivalently to the current lot on known samples before being used for clinical testing.

Question 3

Which document describes the step-by-step procedure for performing a test, including safety precautions, reagents, and interpretation criteria?

Accepted Answer

Standard Operating Procedure (SOP)

Answer

SOPs provide detailed, validated instructions for performing laboratory procedures consistently and are required by CLIA and accreditation standards.

Question 4

What is the significance of turnaround time (TAT) monitoring in a deceased donor histocompatibility laboratory?

Accepted Answer

Rapid TAT is critical because organs have limited viability and allocation decisions cannot wait

Answer

Deceased donor typing, crossmatching, and antibody results must be reported rapidly because organ ischemia time is limited and allocation is time-critical.

Question 5

What is 'delta check' monitoring in the histocompatibility laboratory?

Accepted Answer

Comparing a current patient result to a previous result to flag unexpected changes that may indicate an error

Answer

Delta checks compare current test results against a patient's prior results; unexpected changes (e.g., new HLA allele appearing) trigger investigation for specimen mix-up or error.

CHT - Certified Histocompatibility Technologist Practice Test

CHT - Certified Histocompatibility Technologist Practice Test

CHT Laboratory Management & Safety 2