CABT Laboratory Equipment 3

Question 1

What does the term 'dead volume' refer to in the context of automated liquid handling systems?

Accepted Answer

Volume remaining in a well that cannot be aspirated

Answer

Dead volume is the residual liquid that cannot be aspirated from a vessel due to the geometry of the tip and container, and must be accounted for in assay planning.

Question 2

Which electromagnetic spectrum region is used by a spectrophotometer in the 'UV range'?

Accepted Answer

200-400 nm

Answer

The ultraviolet (UV) range spans approximately 200-400 nm, below the visible spectrum, and is commonly used to measure nucleic acids and aromatic amino acids.

Question 3

A biomedical technician is qualifying a new analytical balance. Which weight class is recommended for calibrating Class I analytical balances?

Accepted Answer

Class E2 or E1 weights

Answer

Class E2 or E1 OIML reference weights provide the accuracy required to calibrate Class I analytical balances used in laboratory settings.

Question 4

Which safety feature prevents a centrifuge from operating when the lid is open?

Accepted Answer

Interlock switch

Answer

An interlock switch (lid interlock) is a safety mechanism that disables centrifuge operation whenever the lid is opened, preventing rotor exposure.

Question 5

In a clinical chemistry analyzer, what is the purpose of a cuvette wash station?

Accepted Answer

To remove residual reagents and samples between measurements to prevent carryover

Answer

The cuvette wash station removes residual reagents and sample between reactions to minimize carryover error that could affect subsequent test results.

(CABT) Certified Associate in Biomedical Technology Practice Test

(CABT) Certified Associate in Biomedical Technology Practice Test

CABT Laboratory Equipment 3