BACE Chromatography Techniques

Question 1

What is the stationary phase in column chromatography?

Accepted Answer

The solid matrix packed inside the column

Answer

The stationary phase is the solid matrix (e.g., resin or beads) packed inside the column that interacts with sample components to achieve separation.

Question 2

In size exclusion chromatography (SEC), which molecules elute first?

Accepted Answer

Large molecules that are excluded from the pores

Answer

Large molecules are excluded from the pores of the resin and travel around them, eluting first with the void volume.

Question 3

What does the term 'elution' refer to in chromatography?

Accepted Answer

Releasing and collecting separated components from the column

Answer

Elution is the process of washing separated analytes off the stationary phase so they can be collected as fractions.

Question 4

Which type of chromatography separates proteins based on their affinity for a specific ligand?

Accepted Answer

Affinity chromatography

Answer

Affinity chromatography uses a ligand bound to the stationary phase that specifically binds the target molecule, allowing highly selective purification.

Question 5

What is the mobile phase in high-performance liquid chromatography (HPLC)?

Accepted Answer

The liquid solvent pumped through the column

Answer

In HPLC, the mobile phase is the liquid solvent mixture that carries the sample through the stationary phase under high pressure.

BACE - Biotechnician Assistant Credentialing Exam Practice Test

BACE - Biotechnician Assistant Credentialing Exam Practice Test

BACE Chromatography Techniques