BACE Chromatography Techniques

Question 1

What is the stationary phase in column chromatography?

Accepted Answer

The solid matrix packed inside the column

Answer

The mobile solvent flowing through the column

Answer

The sample loaded onto the column

Answer

The eluate collected at the bottom

Question 2

In size exclusion chromatography (SEC), which molecules elute first?

Accepted Answer

Large molecules that are excluded from the pores

Answer

Small molecules that enter the pores

Answer

Molecules of intermediate size

Answer

Charged molecules regardless of size

Question 3

What does the term 'elution' refer to in chromatography?

Accepted Answer

Releasing and collecting separated components from the column

Answer

Loading the sample onto the column

Answer

Washing away unwanted contaminants

Answer

Equilibrating the column with buffer

Question 4

Which type of chromatography separates proteins based on their affinity for a specific ligand?

Accepted Answer

Affinity chromatography

Answer

Ion exchange chromatography

Answer

Hydrophobic interaction chromatography

Answer

Gel filtration chromatography

Question 5

What is the mobile phase in high-performance liquid chromatography (HPLC)?

Accepted Answer

The liquid solvent pumped through the column

Answer

The column packing material

Answer

The detector used to monitor separation

Answer

The sample injection loop

Question 6

In ion exchange chromatography, a cation exchange resin carries which charge?

Accepted Answer

Negative charge to attract cations

Answer

Positive charge to attract anions

Answer

No charge; it separates by size

Answer

Variable charge depending on pH

Question 7

What is the purpose of equilibrating a chromatography column before loading a sample?

Accepted Answer

To set the column's pH and ionic conditions to match the sample

Answer

To elute any previously bound proteins

Answer

To pack the resin more tightly

Answer

To sterilize the column before use