B.Pharm. Bachelor of Pharmacy Bachelor of Pharmacy (B.Pharm.) Biochemistry 4

Question 1

In oxidative phosphorylation, the proton gradient across the inner mitochondrial membrane drives ATP synthesis via which complex?

Accepted Answer

Complex V (ATP synthase)

Answer

Complex V (ATP synthase/F0F1-ATPase) harnesses the proton-motive force to phosphorylate ADP to ATP through rotary catalysis.

Question 2

Transamination reactions require which coenzyme derived from vitamin B6?

Accepted Answer

Pyridoxal phosphate (PLP)

Answer

Pyridoxal phosphate forms a Schiff base with the amino acid substrate and shuttles the amino group between donors and α-ketoglutarate in transamination.

Question 3

Which metabolic pathway is exclusively located in the cytosol of eukaryotic cells?

Accepted Answer

Glycolysis

Answer

All ten enzymatic steps of glycolysis occur in the cytoplasm; the other pathways listed are mitochondrial.

Question 4

What is the role of feedback inhibition by CTP on aspartate transcarbamoylase (ATCase) in pyrimidine biosynthesis?

Accepted Answer

It inhibits ATCase to prevent overproduction of pyrimidines

Answer

CTP, the end product of pyrimidine synthesis, allosterically inhibits ATCase at the regulatory subunit, providing classic feedback regulation.

Question 5

Which lipoprotein is primarily responsible for transporting dietary triglycerides from the intestine to peripheral tissues?

Accepted Answer

Chylomicrons

Answer

Chylomicrons are assembled in intestinal enterocytes and transport exogenous dietary triglycerides via lymph into the bloodstream to peripheral tissues.

B.Pharm. Bachelor of Pharmacy Practice Test

B.Pharm. Bachelor of Pharmacy Practice Test

B.Pharm. Bachelor of Pharmacy Bachelor of Pharmacy (B.Pharm.) Biochemistry 4