B.Pharm. Bachelor of Pharmacy Bachelor of Pharmacy (B.Pharm.) Biochemistry 2

Question 1

Which coenzyme is required for the oxidative decarboxylation of pyruvate to acetyl-CoA?

Accepted Answer

Thiamine pyrophosphate

Answer

Thiamine pyrophosphate (TPP) is the essential coenzyme for the pyruvate dehydrogenase complex that catalyzes decarboxylation of pyruvate.

Question 2

In the urea cycle, which amino acid donates both nitrogen atoms that appear in urea?

Accepted Answer

Aspartate and ammonia

Answer

One nitrogen in urea comes from free ammonia (via carbamoyl phosphate) and the second comes from aspartate.

Question 3

Which enzyme is rate-limiting in cholesterol biosynthesis?

Accepted Answer

HMG-CoA reductase

Answer

HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate and is the committed, rate-limiting step in cholesterol synthesis.

Question 4

The Cori cycle transfers energy from the liver to working muscles in what chemical form?

Accepted Answer

Glucose

Answer

The liver converts lactate (from muscles) back to glucose via gluconeogenesis and releases it into the blood for muscle use.

Question 5

Which type of RNA carries amino acids to the ribosome during translation?

Accepted Answer

tRNA

Answer

Transfer RNA (tRNA) carries specific amino acids to the ribosome and matches them to codons on mRNA via its anticodon loop.

B.Pharm. Bachelor of Pharmacy Practice Test