ASCP Phlebotomy

Question 1

What is the recommended angle of needle insertion during routine venipuncture?

Accepted Answer

15–30 degrees

Answer

A 15–30 degree angle allows the needle to enter the vein lumen without penetrating the posterior wall.

Question 2

Which vein is the preferred site for routine venipuncture in the antecubital fossa?

Accepted Answer

Median cubital vein

Answer

The median cubital vein is preferred because it is large, well-anchored, and least likely to roll or be painful.

Question 3

How long should a tourniquet remain applied during venipuncture to avoid hemoconcentration?

Accepted Answer

Less than 1 minute

Answer

Prolonged tourniquet use beyond 1 minute causes venous stasis and hemoconcentration, falsely elevating analytes such as proteins and potassium.

Question 4

What is the correct order of draw when collecting multiple tubes via a single venipuncture?

Accepted Answer

Blood cultures → citrate → serum/SST → heparin → EDTA → fluoride

Answer

The CLSI-recommended order of draw minimizes additive carryover: blood cultures first (sterility), then citrate, serum, heparin, EDTA, and fluoride/oxalate.

Question 5

Which antiseptic is routinely used to cleanse the venipuncture site before needle insertion?

Accepted Answer

70% isopropyl alcohol

Answer

70% isopropyl alcohol is the standard site antiseptic for routine venipuncture and must be allowed to air-dry before needle insertion.

ASCP Practice Test

ASCP Practice Test

ASCP Phlebotomy