Laboratory Practice Flashcards
7 cards from real STP practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 7 Laboratory Practice flashcards as text
When a chemical spill occurs on the skin in a laboratory, what is the FIRST action to take?
Answer: Flush the affected area with large amounts of water for at least 15 minutes
Immediate and prolonged flushing with water dilutes and removes the chemical before it can cause further injury.
Which label element is NOT required on a secondary container of a hazardous chemical in a US laboratory under OSHA's Hazard Communication Standard?
Answer: Manufacturer's full street address
Secondary container labels require a product identifier and hazard warnings but do not need the manufacturer's full street address.
A laboratory worker notices their pipette calibration check is outside the acceptable tolerance range. What should they do?
Answer: Remove the pipette from service, label it 'OUT OF SERVICE,' and report it for recalibration
Equipment outside calibration tolerance must be removed from service to prevent erroneous results and ensure data integrity.
What is the purpose of a Material Safety Data Sheet (SDS) Section 8?
Answer: Exposure controls and personal protective equipment requirements
SDS Section 8 (Exposure Controls/Personal Protection) specifies occupational exposure limits and required PPE.
In a cell culture laboratory, what practice BEST prevents cross-contamination between cell lines?
Answer: Changing gloves and decontaminating the hood between handling different cell lines
Changing gloves and decontaminating surfaces between cell lines prevents transfer of cells, mycoplasma, or other contaminants.
Which of the following is the correct disposal method for a broken glass pipette contaminated with a biohazardous material?
Answer: Biohazardous sharps container
Sharp objects contaminated with biohazardous materials must go into a biohazardous sharps container to address both the puncture risk and biological hazard.
A scientist is setting up a PCR experiment and finds that the positive control failed to amplify. What does this indicate?
Answer: There is likely a problem with the PCR reagents, thermal cycler, or protocol
A failed positive control indicates a technical failure in the assay itself, meaning results from the experimental samples cannot be interpreted.