SAP Drug & Alcohol Testing Procedures

Question 1

What is the minimum urine specimen volume required for a valid DOT drug test collection?

Accepted Answer

45 mL

Answer

Under 49 CFR Part 40, a collector must obtain at least 45 mL of urine; anything less triggers the shy bladder protocol.

Question 2

What temperature range must a freshly voided urine specimen fall within to be considered valid under DOT regulations?

Accepted Answer

90–100°F (32–38°C)

Answer

49 CFR Part 40 requires the specimen temperature to be between 90°F and 100°F (32–38°C) when measured within 4 minutes of collection.

Question 3

Which of the following correctly lists the five drug categories tested in a standard DOT urine drug test?

Accepted Answer

Marijuana, cocaine, opioids, PCP, amphetamines

Answer

The DOT 5-panel test screens for marijuana (THC), cocaine, opioids, phencyclidine (PCP), and amphetamines, as specified in 49 CFR Part 40.

Question 4

At what blood alcohol concentration (BAC) level is a DOT-regulated employee considered to have an alcohol test violation?

Accepted Answer

0.04 or greater

Answer

Under 49 CFR Part 40, a confirmed BAC of 0.04 or greater constitutes an alcohol test violation and prohibits performance of safety-sensitive duties.

Question 5

Under DOT regulations, when is a urine drug test collection required to be conducted under direct observation?

Accepted Answer

For all return-to-duty and follow-up tests

Answer

49 CFR Part 40.67 mandates direct observation for all return-to-duty and follow-up collections to prevent tampering after a prior violation.

(SAP) DOT Certified Substance Abuse Professional Practice Test