Phlebotomy Test National Center for Competency Testing 3 — Questions and Answers
Question 1: Which tube color is used for collecting specimens requiring EDTA as an anticoagulant?
- Red
- Green
- Lavender (Correct answer)
- Light blue
Correct answer: Lavender
Lavender (purple) top tubes contain EDTA and are used for hematology tests such as a complete blood count (CBC).
Question 2: What is the primary purpose of inverting evacuated tubes after collection?
- To warm the specimen for faster processing
- To mix the blood with the tube's additive (Correct answer)
- To prevent the stopper from loosening
- To settle red blood cells to the bottom
Correct answer: To mix the blood with the tube's additive
Gentle inversion mixes blood with the additive (anticoagulant or clot activator) to ensure proper specimen preparation.
Question 3: A patient is identified as a 'difficult stick.' Which site is an acceptable alternative to the antecubital fossa?
- Femoral vein
- Jugular vein
- Hand or wrist veins (Correct answer)
- Ankle veins in all patients
Correct answer: Hand or wrist veins
Hand or wrist veins are an acceptable alternative when antecubital veins are inaccessible; ankle veins require physician order.
Question 4: Which condition requires a phlebotomist to use a smaller gauge needle and gentle aspiration?
- Polycythemia vera
- Dehydration with collapsed veins (Correct answer)
- Thrombocytosis
- Leukocytosis
Correct answer: Dehydration with collapsed veins
Dehydrated patients often have fragile, collapsed veins that require a smaller gauge needle and gentle suction to avoid vein collapse.
Question 5: Under NCCT standards, how should a phlebotomist handle a needlestick injury?
- Squeeze the wound, wash thoroughly, and report immediately to a supervisor (Correct answer)
- Cover with a bandage and continue working
- Apply antiseptic only and document at end of shift
- Notify a supervisor only if bleeding is significant
Correct answer: Squeeze the wound, wash thoroughly, and report immediately to a supervisor
NCCT and OSHA protocols require immediately squeezing the wound, washing with soap and water, and reporting to a supervisor for follow-up care.
Question 6: Which federal regulation governs the handling of bloodborne pathogens and applies directly to phlebotomists?
- CLIA 1988
- OSHA Bloodborne Pathogens Standard 29 CFR 1910.1030 (Correct answer)
- HIPAA Privacy Rule
- FDA 21 CFR Part 606
Correct answer: OSHA Bloodborne Pathogens Standard 29 CFR 1910.1030
OSHA's Bloodborne Pathogens Standard (29 CFR 1910.1030) mandates safety practices to protect healthcare workers from exposure to infectious materials.
Question 7: What is the correct angle range for needle insertion during routine venipuncture?
- 5–10 degrees
- 15–30 degrees (Correct answer)
- 35–45 degrees
- 50–60 degrees
Correct answer: 15–30 degrees
A 15–30 degree angle allows the needle to enter the vein cleanly while minimizing the risk of passing through it.
Which tube color is used for collecting specimens requiring EDTA as an anticoagulant?