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Special Collection Procedures Flashcards

7 cards from real Phlebotomy Test practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.

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  1. When collecting blood cultures, how should the venipuncture site be prepared to minimize contamination?

    Answer: Chlorhexidine gluconate or iodine tincture applied in concentric circles and allowed to dry

    Chlorhexidine or iodine-based antiseptics applied in an outward concentric-circle motion and allowed to fully dry provide the best reduction in skin flora.

  2. In a 2-bottle blood culture set, which bottle should be inoculated first when using a winged blood collection set (butterfly needle)?

    Answer: Aerobic bottle first

    When using a butterfly set, the tubing contains air; inoculating the aerobic bottle first prevents that air from entering the anaerobic bottle.

  3. Which type of specimen is required for a sweat chloride test used to diagnose cystic fibrosis?

    Answer: Sweat collected via pilocarpine iontophoresis onto gauze or filter paper

    The pilocarpine iontophoresis method stimulates localized sweating, and the collected sweat is analyzed for chloride concentration.

  4. A glucose tolerance test (GTT) timed specimen drawn at the 2-hour interval must be collected within how many minutes of the scheduled time to be valid?

    Answer: ± 5 minutes

    GTT specimens must be collected within ±5 minutes of the scheduled interval to ensure accurate glucose curve interpretation.

  5. What is the recommended minimum volume of blood to inoculate into each blood culture bottle in an adult collection?

    Answer: 8–10 mL

    Inoculating 8–10 mL per bottle maximizes the sensitivity of blood culture by increasing the chance of detecting low-level bacteremia.

  6. When is an icteric (jaundiced) specimen clinically significant in special collection protocols?

    Answer: It can interfere with spectrophotometric assays, causing falsely elevated or decreased results

    Bilirubin's yellow pigment absorbs light at wavelengths used in many chemistry assays, causing interference and inaccurate results.

  7. Which phlebotomy procedure is most appropriate when a patient requires serial glucose monitoring every hour during a GTT?

    Answer: Capillary fingerstick using a lancet

    Capillary fingersticks minimize patient discomfort and vein damage during the multiple timed collections required for a GTT.