A 10-year-old Quarter Horse gelding presents with a 2-week history of asymmetric hindlimb ataxia, gluteal muscle atrophy, and a head tilt. The owner reports the signs are slowly progressive. Which of the following diagnostic approaches is considered the gold standard for an antemortem diagnosis of Equine Protozoal Myeloencephalitis (EPM)?
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A
Serum antibody testing for Sarcocystis neurona.
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B
Western blot analysis of cerebrospinal fluid (CSF) alone.
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C
Response to a course of antiprotozoal treatment.
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D
Paired serum and CSF antibody testing to demonstrate intrathecal antibody production.