BACE - Biotechnician Assistant Credentialing Laboratory Safety and Regulations Questions and Answers

Question 1

A biotechnician needs to find information on the appropriate first-aid measures to take after accidental skin contact with a chemical. Which section of the Safety Data Sheet (SDS) should they consult?

Accepted Answer

Section 4: First-Aid Measures

Answer

Section 4 of an SDS specifically details the initial care that should be given by untrained responders following exposure to the chemical, covering routes like skin/eye contact, inhalation, and ingestion.

Question 2

A researcher is working with human-derived cell lines, which are considered potentially infectious materials under most circumstances. According to OSHA's Bloodborne Pathogens Standard, which of the following is the MOST appropriate combination of engineering controls and personal protective equipment (PPE)?

Accepted Answer

A Class II Biosafety Cabinet, lab coat, and gloves.

Answer

OSHA considers human cell lines to be potentially infectious materials. A Class II Biosafety Cabinet is an engineering control that protects the user from bioaerosols, the environment, and the sample from contamination. A lab coat and gloves are the minimum required PPE for handling such materials at Biosafety Level 2.

Question 3

A biotechnician has just finished running an agarose gel stained with ethidium bromide, a known mutagen. Which of the following is the proper procedure for disposing of the used gel?

Accepted Answer

Place it in a designated, sealed hazardous waste container for ethidium bromide.

Answer

Ethidium bromide is a hazardous chemical waste due to its mutagenic properties and must be segregated for special disposal. It should not be placed in regular trash or autoclaved, as heat does not neutralize its chemical hazard. It must be collected in a clearly labeled, sealed hazardous waste container for professional disposal.

Question 4

A technician prepares a diluted working solution of a hazardous chemical in a spray bottle for daily use. The original stock bottle has a complete GHS label. What is the MINIMUM information required by OSHA on the label for this new 'secondary container'?

Accepted Answer

The chemical identity and words, pictures, or symbols indicating the hazard.

Answer

According to OSHA's Hazard Communication Standard, secondary containers must, at a minimum, be labeled with the identity of the hazardous chemical and general information regarding its hazards using words, pictures, or symbols. While a full GHS label is an option, it is not the minimum requirement.

Question 5

The OSHA Hazard Communication Standard (HCS) is primarily designed to ensure that:

Accepted Answer

the hazards of all chemicals are evaluated and this information is communicated to employees.

Answer

The core purpose of the Hazard Communication Standard is to ensure that employers evaluate chemical hazards and that employees are informed about them through a comprehensive program that includes container labeling, safety data sheets (SDS), and training. The hepatitis B vaccine is covered under the Bloodborne Pathogens Standard.

BACE - Biotechnician Assistant Credentialing Exam Practice Test

BACE - Biotechnician Assistant Credentialing Exam Practice Test

BACE - Biotechnician Assistant Credentialing Laboratory Safety and Regulations Questions and Answers