MRO Drug Testing Procedures 2 — Questions and Answers
Question 1: What is the minimum urine specimen volume required for a DOT federal workplace drug test?
- 30 mL (Correct answer)
- 45 mL
- 60 mL
- 90 mL
Correct answer: 30 mL
DOT regulations require a minimum of 30 mL of urine to complete a federal workplace drug test.
Question 2: Which document is used to document chain of custody for a federal workplace urine drug test?
- DEA Form 222
- Federal Drug Testing Custody and Control Form (CCF) (Correct answer)
- SAMHSA-1 Form
- MRO Verification Report
Correct answer: Federal Drug Testing Custody and Control Form (CCF)
The Federal CCF is the required form for maintaining chain of custody in all DOT-mandated urine drug tests.
Question 3: If a donor cannot provide an adequate specimen on the first attempt, how long must the collector allow the donor to try again?
- 15 minutes
- Up to 3 hours (Correct answer)
- 30 minutes
- Until the next scheduled workday
Correct answer: Up to 3 hours
Under DOT rules, the collector must allow the donor up to 3 hours to provide a sufficient specimen before documenting a refusal.
Question 4: When a direct observation collection is required, who may observe the donor provide the specimen?
- Any trained collector regardless of gender
- A collector of the same gender as the donor (Correct answer)
- A licensed medical professional only
- The donor's direct supervisor
Correct answer: A collector of the same gender as the donor
DOT regulations require that the observer during a direct observation collection must be the same gender as the donor.
Question 5: What is the acceptable temperature range for a urine specimen when presented to the collector?
- 90–100°F (32–38°C) (Correct answer)
- 95–105°F (35–41°C)
- 98–106°F (37–41°C)
- 86–98°F (30–37°C)
Correct answer: 90–100°F (32–38°C)
A valid specimen must read between 90°F and 100°F on the temperature strip within 4 minutes of collection.
Question 6: What action must a collector take if a specimen temperature falls outside the acceptable range?
- Discard the specimen and end the collection
- Document the discrepancy on the CCF and collect a second specimen under direct observation (Correct answer)
- Accept the specimen and note the temperature discrepancy only
- Immediately report the issue to the MRO before proceeding
Correct answer: Document the discrepancy on the CCF and collect a second specimen under direct observation
An out-of-range temperature triggers a CCF notation and a second collection under direct observation to detect possible substitution.
Question 7: At what point during the collection process must the collector seal the specimen bottles?
- In a private collection area away from the donor
- In the presence of the donor (Correct answer)
- After the donor has left the collection facility
- Only when witnessed by the MRO
Correct answer: In the presence of the donor
Sealing the specimen bottles in the donor's presence maintains chain of custody integrity and prevents tampering allegations.
What is the minimum urine specimen volume required for a DOT federal workplace drug test?