MLPAO Specimen Collection and Procurement Questions and Answers 2

Question 1

What is the recommended angle for needle insertion during standard adult antecubital venipuncture?

Accepted Answer

15-30 degrees

Answer

5-10 degrees

Answer

45-60 degrees

Answer

90 degrees (perpendicular)

Question 2

Which vein is considered the preferred primary site for adult venipuncture and why?

Accepted Answer

Median cubital vein — it is large, well-anchored, and has few nearby nerves and arteries

Answer

Basilic vein — it is the largest and most accessible

Answer

Cephalic vein — it is most visible in all patients

Answer

Dorsal hand veins — they are the safest and most accessible

Question 3

What action should be taken if a hematoma begins to form during venipuncture?

Accepted Answer

Immediately release the tourniquet, withdraw the needle, and apply firm pressure

Answer

Continue collection and massage the site to disperse the hematoma

Answer

Apply the tourniquet more tightly and continue

Answer

Redirect the needle to a different vein in the same arm

Question 4

For a glucose tolerance test (GTT), a 75g oral glucose challenge is administered. When is the post-load blood specimen collected for a standard 2-hour OGTT?

Accepted Answer

120 minutes (2 hours) after glucose ingestion

Answer

30 minutes after glucose ingestion

Answer

60 minutes after glucose ingestion

Answer

90 minutes after glucose ingestion

Question 5

What is the function of lithium heparin in a green-top blood collection tube?

Accepted Answer

It inhibits thrombin and activated factor X to prevent clotting while preserving cellular morphology

Answer

It prevents bacterial growth in the specimen

Answer

It chelates calcium to prevent coagulation

Answer

It inhibits glycolysis to preserve glucose levels

Question 6

A patient is undergoing therapeutic phlebotomy for hereditary hemochromatosis. What is the standard volume of blood removed per session in adult patients?

Accepted Answer

450-500 mL

Answer

100-200 mL

Answer

250-350 mL

Answer

700-900 mL