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FREE Medical Laboratory Technician Clinical Chemistry Procedures Questions and Answers Flashcards

6 cards from real Medical Laboratory Technician  Exam practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.

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  1. Which enzyme is most commonly measured to assess acute pancreatitis in clinical chemistry?

    Answer: Lipase

    Lipase is the preferred enzyme marker for acute pancreatitis due to its high sensitivity and specificity for pancreatic tissue damage.

  2. What is the principle behind the Jaffe reaction used in clinical chemistry laboratories?

    Answer: Creatinine reacts with alkaline picrate to form a red-orange complex

    The Jaffe reaction measures creatinine by forming a red-orange chromogen when creatinine reacts with picric acid in an alkaline solution.

  3. In the enzymatic method for glucose measurement, what does hexokinase catalyze?

    Answer: Phosphorylation of glucose to glucose-6-phosphate

    Hexokinase catalyzes the phosphorylation of glucose using ATP to produce glucose-6-phosphate, which is then further reacted to generate a measurable signal.

  4. Which specimen type is preferred for measuring ionized calcium in clinical chemistry?

    Answer: Anaerobic whole blood collected in heparin

    Anaerobic heparinized whole blood is preferred because exposure to air increases pH, which shifts calcium binding to albumin and falsely lowers ionized calcium.

  5. What does an elevated serum AST-to-ALT ratio greater than 2:1 typically suggest?

    Answer: Alcoholic liver disease

    An AST-to-ALT ratio greater than 2:1 is a classic pattern associated with alcoholic liver disease due to alcohol-induced mitochondrial damage releasing more AST.

  6. Which electrolyte measurement is most affected by hemolysis of a blood specimen?

    Answer: Potassium

    Potassium is present in much higher concentrations inside red blood cells than in plasma, so hemolysis causes a significant falsely elevated potassium result.