Microbiology for Sterile Processing Flashcards

Why is Geobacillus stearothermophilus used as the BI organism for steam sterilization?

Answer: Its spores are highly resistant to moist heat, making it a reliable challenge organism

G. stearothermophilus spores are exceptionally resistant to steam, requiring conditions beyond normal sterilization for kill.

What is a biofilm and why is it significant in sterile processing?

Answer: Complex microbial community in a protective matrix that is extremely resistant to cleaning and sterilization

Biofilms are microbial communities encased in a protective matrix making them extremely difficult to remove.

What is the significance of bacterial endospores in sterilization?

Answer: The most resistant form of microbial life and the benchmark for sterilization efficacy

Endospores are the most resistant microbial form, capable of surviving extreme conditions.

What is the chain of infection and how does SPD break it?

Answer: Sequence of six elements required for infection; SPD breaks it by eliminating transmission through sterile instruments

SPD breaks the chain by eliminating contaminated instruments as a mode of transmission through proper cleaning and sterilization.

What is the difference between transient and resident microflora on hands?

Answer: Transient flora are recently acquired and easily removed; resident flora are permanent skin inhabitants

Transient flora are temporary organisms easily removed by hand washing; resident flora are permanent. Both can contaminate sterile items.

What are prions and why are they a unique challenge?

Answer: Misfolded proteins that are extremely resistant to standard sterilization methods

Prions are infectious misfolded proteins extraordinarily resistant to conventional sterilization and disinfection.