Biology: Cellular Metabolism Flashcards
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The pyruvate dehydrogenase complex converts pyruvate into which molecule entering the citric acid cycle?
Answer: Acetyl-CoA
Pyruvate is oxidatively decarboxylated to acetyl-CoA, releasing CO2 and producing NADH.
How many CO2 molecules are released per turn of the citric acid cycle?
Answer: 2
Isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase each release one CO2 per turn.
Which molecule combines with acetyl-CoA to form citrate?
Answer: Oxaloacetate
Citrate synthase condenses acetyl-CoA with oxaloacetate to begin the cycle.
Per turn of the citric acid cycle, how many NADH molecules are produced?
Answer: 3
Three NADH are generated at the isocitrate, alpha-ketoglutarate, and malate dehydrogenase steps.
Which high-energy molecule is produced directly by substrate-level phosphorylation in the citric acid cycle?
Answer: GTP
Succinyl-CoA synthetase produces GTP (or ATP) by substrate-level phosphorylation.
In which cellular compartment does the citric acid cycle occur?
Answer: Mitochondrial matrix
All citric acid cycle enzymes reside in the mitochondrial matrix, except succinate dehydrogenase.
Which citric acid cycle enzyme is also a component of the electron transport chain (Complex II)?
Answer: Succinate dehydrogenase
Succinate dehydrogenase is embedded in the inner membrane and feeds FADH2 electrons directly into Complex II.