CTBS Tissue Recovery 2 — Questions and Answers
Question 1: What is the maximum acceptable warm ischemia time for most musculoskeletal tissue donors?
- 6 hours (Correct answer)
- 12 hours
- 24 hours
- 48 hours
Correct answer: 6 hours
Warm ischemia time for musculoskeletal tissue should generally not exceed 6 hours after asystole to maintain tissue viability.
Question 2: Which of the following is a contraindication to tissue donation due to risk of disease transmission?
- History of appendectomy
- Active systemic bacterial sepsis (Correct answer)
- Controlled type 2 diabetes
- Prior broken bone
Correct answer: Active systemic bacterial sepsis
Active systemic bacterial sepsis is a contraindication to tissue donation because it poses a risk of transmitting infection to recipients.
Question 3: When recovering tissue from a donor with unknown HIV status, which precaution is MOST important during the recovery procedure?
- Use double gloves and face shield (Correct answer)
- Avoid recovering any tissue
- Proceed only if NAT testing is complete
- Request next-of-kin confirmation of HIV status
Correct answer: Use double gloves and face shield
Standard and enhanced personal protective equipment including double gloving and face shields must be used when infectious status is unknown to protect recovery staff.
Question 4: What documentation must accompany recovered tissue from the recovery site to the processing facility?
- Chain of custody form and donor chart (Correct answer)
- Only the death certificate
- Recipient matching form
- Insurance authorization letter
Correct answer: Chain of custody form and donor chart
A chain of custody form along with the donor chart ensures traceability and integrity of the tissue from recovery through processing.
Question 5: Which body area is typically used for harvesting saphenous vein grafts during tissue recovery?
- Upper arm
- Medial thigh and lower leg (Correct answer)
- Axillary region
- Abdominal wall
Correct answer: Medial thigh and lower leg
The saphenous vein runs along the medial thigh and lower leg and is the standard harvest site for venous allografts.
Question 6: What is the primary purpose of maintaining detailed recovery logs during tissue procurement?
- To bill insurance companies
- To ensure regulatory compliance and traceability (Correct answer)
- To schedule future recoveries
- To notify next of kin of timing
Correct answer: To ensure regulatory compliance and traceability
Detailed recovery logs support regulatory compliance under FDA 21 CFR Part 1271 and enable full traceability in the event of a recall or adverse event.
Question 7: When is it acceptable to recover tissue from a donor who has received cardiopulmonary resuscitation (CPR)?
- Never; CPR automatically disqualifies a donor
- Only if resuscitation lasted less than 30 minutes and was successful
- After evaluating time of death, warm ischemia, and physical tissue condition (Correct answer)
- Only if approved by the medical examiner
Correct answer: After evaluating time of death, warm ischemia, and physical tissue condition
CPR itself is not an absolute contraindication; the recovery team must evaluate ischemia time, physical condition of tissue, and other eligibility criteria.
CTBS Tissue Recovery 2
What is the maximum acceptable warm ischemia time for most musculoskeletal tissue donors?