CTBS Processing Techniques 4

Question 1

Which type of bone graft has both osteogenic AND osteoinductive AND osteoconductive properties?

Accepted Answer

Fresh autograft

Answer

Fresh autograft is the only graft type possessing all three bone healing properties: osteogenic (viable cells), osteoinductive (BMPs), and osteoconductive (scaffold), making it the gold standard.

Question 2

In tissue bank processing, what is the purpose of performing a 'gram stain' during microbiological testing?

Accepted Answer

To differentiate between Gram-positive and Gram-negative bacteria for early contamination detection

Answer

Gram staining rapidly differentiates Gram-positive from Gram-negative bacteria based on cell wall composition, providing early indication of bacterial contamination during processing.

Question 3

What is the primary concern with using high-dose gamma irradiation (> 25 kGy) to sterilize cortical bone allografts?

Accepted Answer

Significant reduction in compressive and torsional mechanical strength

Answer

High-dose gamma irradiation causes significant free radical-mediated damage to collagen cross-links, substantially reducing the compressive strength, fatigue resistance, and torsional properties of cortical bone.

Question 4

During processing of amniotic membrane allografts, which property must be preserved to maintain its clinical utility as a wound cover?

Accepted Answer

Intact basement membrane and collagen matrix with growth factors

Answer

The intact basement membrane and collagen matrix containing growth factors (EGF, FGF, TGF-β) are essential for the amniotic membrane's anti-inflammatory, anti-scarring, and wound healing properties.

Question 5

What does the term 'terminal sterilization' mean in tissue processing?

Accepted Answer

Sterilization applied to the final packaged product after all processing steps

Answer

Terminal sterilization refers to the application of a sterilization process (e.g., gamma irradiation, electron beam) to the tissue in its final, sealed packaging after all other processing steps are complete.

(CTBS) Certified Tissue Bank Specialist Practice Test

(CTBS) Certified Tissue Bank Specialist Practice Test

CTBS Processing Techniques 4