CTBS Processing Techniques 2

Question 1

Which solution is most commonly used for terminal sterilization of bone allografts via chemical means?

Accepted Answer

Peracetic acid (PAA)

Answer

Peracetic acid is widely used for terminal sterilization of bone allografts because it is sporicidal, leaves minimal toxic residues, and is compatible with bone matrix.

Question 2

What is the primary purpose of demineralization in bone allograft processing?

Accepted Answer

To expose bone morphogenetic proteins and enhance osteoinductivity

Answer

Demineralization removes calcium phosphate mineral to expose bone morphogenetic proteins (BMPs) embedded in the collagen matrix, significantly enhancing the osteoinductive potential of the graft.

Question 3

During skin allograft processing, which step is performed to reduce bioburden prior to cryopreservation?

Accepted Answer

Antibiotic solution soaking

Answer

Skin allografts are typically soaked in antibiotic solution cocktails (e.g., gentamicin, vancomycin) to reduce bacterial bioburden before cryopreservation.

Question 4

What does the term 'bioburden' refer to in the context of tissue processing?

Accepted Answer

The number of viable microorganisms present on or in a tissue before sterilization

Answer

Bioburden refers to the total number of viable microorganisms present on or in a tissue before sterilization, and monitoring it is critical to validating sterilization processes.

Question 5

Which processing technique is specifically designed to preserve the viability of chondrocytes in cartilage allografts?

Accepted Answer

Controlled-rate cryopreservation with DMSO

Answer

Controlled-rate cryopreservation using DMSO (dimethyl sulfoxide) as a cryoprotectant is used to maintain chondrocyte viability in osteochondral and cartilage allografts.

(CTBS) Certified Tissue Bank Specialist Practice Test

(CTBS) Certified Tissue Bank Specialist Practice Test

CTBS Processing Techniques 2