Specimen Collection & Handling Flashcards

Which of the following best describes the proper technique for mixing an EDTA tube after collection?

Answer: Invert gently 8–10 times

EDTA tubes should be gently inverted 8–10 times to thoroughly mix the anticoagulant with blood without causing hemolysis.

A urine specimen for culture must be collected using which method to avoid contamination?

Answer: Midstream clean-catch into a sterile cup

Midstream clean-catch technique minimizes urethral flora contamination for accurate urine culture results.

Which specimen type is collected in a yellow (ACD) tube?

Answer: Whole blood for blood banking and HLA typing

Yellow ACD (acid citrate dextrose) tubes are used for whole blood specimens in blood banking, HLA phenotyping, and paternity testing.

A specimen labeled 'STAT' is received in the lab 45 minutes after collection with no temperature notation. What should the laboratory assistant do?

Answer: Check for visible hemolysis and contact the ordering unit

The assistant should assess the specimen for hemolysis or other integrity issues and contact the ordering unit to clarify handling before processing.

What is the minimum volume typically required in a sodium citrate tube to maintain the correct blood-to-anticoagulant ratio?

Answer: At least 90% of the stated tube volume (no more than 10% underfilled)

Sodium citrate tubes must be filled to at least 90% of capacity to maintain the 9:1 blood-to-anticoagulant ratio required for valid coagulation testing.

Which needle gauge is most commonly used for routine adult venipuncture?

Answer: 21–22 gauge

21–22 gauge needles are standard for adult venipuncture, balancing flow rate with patient comfort and minimal hemolysis.

When collecting a blood specimen from a patient receiving IV fluids, which site is preferred?

Answer: The opposite arm from the IV site

Blood should be drawn from the opposite arm of an IV site to prevent dilution or contamination of the specimen with infused fluids.