REE CMLA Laboratory Safety & Procedures Flashcards

Which action is NOT acceptable when working in a laboratory with open flames?

Answer: Wearing loose-fitting synthetic clothing

Loose-fitting synthetic fabrics ignite easily and melt onto the skin, creating severe burn injuries near open flames.

An eyewash station must be activated and flushed for how long during an eye chemical exposure?

Answer: 15–20 minutes

ANSI Z358.1 requires continuous flushing of the eyes for a minimum of 15 minutes to adequately remove chemical contaminants.

What is the purpose of a biological safety cabinet (BSC) Class II Type A2?

Answer: Protects personnel, product, and the environment

Class II Type A2 BSCs provide triple protection — personnel, product, and environment — through HEPA-filtered inflow and exhaust.

Which waste stream is used for blood-soaked materials and cultures of infectious agents?

Answer: Regulated medical/biohazardous waste (red bag)

Blood-soaked materials and infectious cultures are regulated medical waste and must be placed in red biohazard bags for proper disposal.

OSHA's Bloodborne Pathogen Standard requires that employers offer hepatitis B vaccination to at-risk employees:

Answer: Within 10 working days of initial assignment

OSHA 29 CFR 1910.1030 mandates that Hep B vaccination be offered within 10 working days of initial assignment to at-risk employees.

When pipetting corrosive or infectious liquids, laboratory personnel must use:

Answer: Mechanical pipetting devices only

Mouth pipetting is absolutely prohibited; mechanical pipetting devices (bulbs, electronic pipettors) must always be used to prevent ingestion or inhalation.

A laboratory worker notices a gas odor near a Bunsen burner. The FIRST action should be to:

Answer: Turn off the gas supply and ventilate the area

Immediately shutting off the gas supply and ventilating prevents accumulation of flammable gas and eliminates ignition risk.