CLT Laboratory Equipment Operation & Maintenance 2

Question 1

When performing a Coulter principle cell counter QC check, which parameter most directly reflects RBC volume distribution?

Accepted Answer

RDW

Answer

RDW (Red Cell Distribution Width) directly measures the variability in RBC volume, making it the key parameter for assessing RBC volume distribution on Coulter principle analyzers.

Question 2

A centrifuge rotor shows visible pitting after routine cleaning. The technologist should:

Accepted Answer

Remove it from service and notify the supervisor immediately

Answer

Pitting in a centrifuge rotor indicates metal fatigue and potential for catastrophic failure; the rotor must be removed from service immediately as a safety measure.

Question 3

Which component of a spectrophotometer is responsible for isolating a specific wavelength of light?

Accepted Answer

Monochromator

Answer

The monochromator (using a prism, diffraction grating, or filter) isolates the desired wavelength of light from a broad-spectrum source for accurate absorbance measurement.

Question 4

A biosafety cabinet (BSC) Class II Type A2 is PRIMARILY designed to protect:

Accepted Answer

Personnel, product, and environment

Answer

Class II Type A2 BSCs provide triple protection — personnel (via inward airflow), product (via HEPA-filtered downflow), and environment (via HEPA-filtered exhaust).

Question 5

During autoclave validation, biological indicators (BIs) containing Geobacillus stearothermophilus spores are used because these spores:

Accepted Answer

Represent the most heat-resistant organisms

Answer

G. stearothermophilus spores are the gold standard BI for steam sterilization because they are among the most heat-resistant organisms, ensuring sterilization efficacy when they are killed.

(CLT) Clinical Laboratory Technologist Certification Practice Test

(CLT) Clinical Laboratory Technologist Certification Practice Test

CLT Laboratory Equipment Operation & Maintenance 2