Laboratory Equipment Operation & Maintenance Flashcards
7 cards from real CLT practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 7 Laboratory Equipment Operation & Maintenance flashcards as text
The linearity verification for a glucose analyzer should be performed using:
Answer: Commercial linearity materials spanning the reportable range
Commercial linearity materials with certified target values spanning the analytical measurement range (AMR) are used to verify that the analyzer produces results proportional to analyte concentration across its full range.
A pH meter requires calibration with buffers at two points before use. This is done to:
Answer: Correct for both the offset and slope of the electrode response
Two-point calibration corrects the offset (zero intercept) and the slope of the glass electrode's response curve, ensuring accurate pH readings across the measurement range.
When a laboratory pipette fails its gravimetric verification, the technologist should:
Answer: Tag it out of service and send it for repair or recalibration
A pipette that fails gravimetric verification must be taken out of service immediately and sent for professional recalibration or repair to prevent analytical errors.
The function of the sheath fluid in a flow cytometer is to:
Answer: Hydrodynamically focus cells into a single-file stream
Sheath fluid surrounds the sample stream in a flow cytometer, using hydrodynamic focusing to align cells single-file through the laser interrogation point for accurate individual cell analysis.
An ion-selective electrode (ISE) analyzer for sodium shows a carryover effect between high and low samples. The MOST likely cause is:
Answer: Insufficient washout volume between samples
Carryover in ISE analyzers is most commonly caused by insufficient wash volume between samples, causing residual analyte from a high sample to contaminate the next low sample.
During preventive maintenance of an automated chemistry analyzer, the probe wash station is cleaned because:
Answer: Protein and salt buildup can impair probe cleansing and cause carryover
Protein deposits and salt crystals accumulating in the probe wash station reduce washing effectiveness, leading to sample-to-sample carryover and inaccurate results.
A hemocytometer is used to manually count cells. The accepted standard dilution for a WBC count using a hemocytometer is:
Answer: 1:20 with acetic acid solution
A 1:20 dilution with 3% acetic acid (Türk's solution) is the standard for manual WBC hemocytometer counts — the acid lyses RBCs while preserving WBCs for counting.