Clinical Chemistry & Biochemical Analysis Flashcards
7 cards from real CLT practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 7 Clinical Chemistry & Biochemical Analysis flashcards as text
Which of the following best describes the Beer-Lambert Law as applied to spectrophotometry in clinical chemistry?
Answer: Absorbance is directly proportional to concentration and path length
Beer-Lambert Law states that absorbance equals the product of molar absorptivity, path length, and concentration, meaning absorbance increases linearly with concentration.
Hemoglobin A1c (HbA1c) reflects average blood glucose control over approximately what time period?
Answer: 8–12 weeks
HbA1c reflects the average plasma glucose over the preceding 8–12 weeks (approximately 3 months), corresponding to the lifespan of red blood cells.
Troponin I or T elevation in a patient with chest pain is most indicative of which condition?
Answer: Acute myocardial infarction
Cardiac troponins I and T are released from injured myocardial cells and are the gold-standard biomarkers for acute myocardial infarction.
Which method is considered the reference (gold standard) for measuring serum cholesterol?
Answer: Abell-Kendall method
The Abell-Kendall method (saponification followed by Liebermann-Burchard colorimetry under controlled conditions) is the CDC reference method for total cholesterol.
An arterial blood gas result shows pH 7.28, pCO2 55 mmHg, and HCO3 26 mEq/L. What is the primary acid-base disturbance?
Answer: Respiratory acidosis
Elevated pCO2 (>45 mmHg) with a low pH indicates respiratory acidosis caused by CO2 retention.
What is the primary purpose of running a calibration curve in clinical chemistry assays?
Answer: To establish the relationship between signal and analyte concentration
A calibration curve plots known standard concentrations against instrument response (absorbance/signal) so that unknown sample concentrations can be calculated by interpolation.
Elevated serum amylase and lipase are most characteristic of which condition?
Answer: Acute pancreatitis
Both amylase and lipase are pancreatic enzymes that are released into the bloodstream during acute pancreatitis, making their elevation diagnostic of this condition.