CFC DNA Analysis & Interpretation 5

Question 1

During autopsy, bone marrow samples are collected for DNA identification of a decomposed victim. Which extraction method is most commonly used for this sample type?

Accepted Answer

Chelex extraction

Answer

Chelex extraction is commonly used for bone marrow and other forensic samples because it is simple, fast, and effective for low-template or degraded samples.

Question 2

What is the significance of Hardy-Weinberg equilibrium (HWE) in forensic DNA statistics?

Accepted Answer

It provides the mathematical basis for calculating genotype frequencies from allele frequencies in a population

Answer

HWE allows forensic analysts to calculate the expected frequency of a genotype (diploid pair) by multiplying individual allele frequencies, foundational to the product rule.

Question 3

A forensic consultant reviews a case where the original analyst used only inclusion/exclusion rather than probabilistic genotyping for a three-person mixture. What concern should the consultant raise?

Accepted Answer

Binary methods may over-include or under-include contributors in complex mixtures, whereas probabilistic methods provide more accurate statistical weight

Answer

Binary inclusion/exclusion methods for complex mixtures can produce misleading results; probabilistic genotyping provides a more scientifically rigorous evaluation.

Question 4

In sexual assault casework, 'differential extraction' is used to separate which two cell populations?

Accepted Answer

Sperm cells and epithelial cells

Answer

Differential extraction uses a controlled lysis procedure to separate sperm cells (male contributor) from epithelial cells (female victim), yielding separate DNA fractions.

Question 5

A forensic examiner is asked whether a DNA profile developed from a crime scene can be used to infer the donor's physical appearance. What is the current state of forensic DNA phenotyping (FDP)?

Accepted Answer

FDP can predict some traits (eye color, hair color, ancestry) with varying accuracy, but has limitations and is not routinely used in court as definitive identification

Answer

Forensic DNA phenotyping can predict some visible characteristics from DNA, but accuracy varies by trait and it is used investigatively rather than for definitive identification.

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(CFC) Certified Forensic Consultant Practice Test

CFC DNA Analysis & Interpretation 5