ASCP Immunology and Serology 2

Question 1

Which complement pathway is initiated by antibody-antigen complexes?

Accepted Answer

Classical pathway

Answer

Alternative pathway

Answer

Lectin (MBL) pathway

Answer

Terminal pathway only

Question 2

The Western blot test for HIV confirmation detects antibodies against which viral proteins?

Accepted Answer

p24 core, gp41 envelope, and gp120/160 envelope proteins

Answer

Viral RNA polymerase subunits

Answer

HLA antigens on lymphocytes

Answer

CD4 receptor complexes

Question 3

Anti-CCP (anti-cyclic citrullinated peptide) antibodies are most specific for:

Accepted Answer

Rheumatoid arthritis

Answer

Systemic lupus erythematosus

Answer

Sjögren's syndrome

Answer

Polymyositis

Question 4

The indirect fluorescent antibody (IFA) test for ANA uses which substrate?

Accepted Answer

HEp-2 (human epithelial) cells

Answer

Sheep red blood cells

Answer

Rabbit liver sections

Answer

Cardiolipin-coated microplates

Question 5

Which marker is used to diagnose infectious mononucleosis caused by Epstein-Barr virus?

Accepted Answer

Monospot test (heterophile antibodies) plus EBV-specific antibodies

Answer

VDRL test

Answer

Anti-HBc IgM

Answer

Cold agglutinins

Question 6

Flow cytometry is used to enumerate CD4+ T cells in HIV patients to:

Accepted Answer

Monitor immune status and guide antiretroviral therapy initiation

Answer

Detect viral RNA load

Answer

Diagnose opportunistic infections

Answer

Measure antibody titers