ALAT Facility Operations and Sanitation 2

Question 1

What temperature must the final rinse water reach in a cage washer for sanitization?

Accepted Answer

180 F (82.2 C)

Answer

100 F (37.8 C)

Answer

140 F (60 C)

Answer

212 F (100 C)

Question 2

What is the correct order of steps in cleaning an animal room?

Accepted Answer

Observe animals, change cages, remove soiled materials, clean surfaces, sanitize, check environment

Answer

Mop first, then change cages

Answer

Check environment, mop, change cages

Answer

Sanitize first, then check animals

Question 3

Why is it important to verify sipper tubes function after placing water bottles on cages?

Accepted Answer

To ensure animals have access to water, since a clogged tube can cause dehydration and death

Answer

To prevent water waste

Answer

To verify temperature

Answer

To measure consumption

Question 4

What is the purpose of biological indicators (BIs) for monitoring autoclave performance?

Accepted Answer

To verify sterilization by testing survival of highly resistant bacterial spores

Answer

To add color for identification

Answer

To measure weight

Answer

To detect chemical contamination

Question 5

What information should be recorded on a cage card?

Accepted Answer

Species, strain, number, sex, birth/receipt date, protocol number, investigator, and special care instructions

Answer

Only the researcher's name

Answer

Only cage change date

Answer

Only room number

Question 6

What is the difference between cleaning, sanitization, and sterilization?

Accepted Answer

Cleaning removes visible soil; sanitization reduces microorganisms to safe levels; sterilization destroys all microbial life including spores

Answer

All the same process

Answer

Sterilization is less effective

Answer

Sanitization only applies to food areas