ALAT Research Procedures and Techniques Questions and Answers Flashcards
7 cards from real ALAT practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 7 ALAT Research Procedures and Techniques Questions and Answers flashcards as text
When collecting a blood sample from a mouse via the retro-orbital sinus, which anesthetic approach is most commonly recommended to minimize pain and distress?
Answer: General anesthesia using isoflurane or injectable agents
Retro-orbital blood collection requires general anesthesia to prevent pain and minimize the risk of ocular trauma.
What is the recommended maximum volume for a single subcutaneous injection in a rat weighing 250 g?
Answer: 2.5 mL
The recommended maximum subcutaneous injection volume in rats is approximately 1–2 mL per site, with up to 5 mL/kg body weight total, equating to ~2.5 mL for a 250 g rat.
A researcher asks you to perform a gavage on a mouse with a compound that has a pH of 2. What is your most important concern?
Answer: Acidic compounds can cause severe esophageal and gastric irritation or injury
Highly acidic compounds can damage the esophageal and gastric mucosa during oral gavage, posing a significant welfare concern.
Which of the following correctly describes the preferred site for intraperitoneal (IP) injection in mice to avoid organ damage?
Answer: Lower left quadrant of the abdomen with the animal's head tilted down
IP injections in mice are given in the lower left quadrant with the head tilted downward to shift organs away and reduce the risk of penetrating the cecum or bladder.
When using isoflurane for rodent anesthesia in a research procedure, what equipment is essential to protect personnel from waste anesthetic gas exposure?
Answer: A scavenging system connected to the anesthesia machine
A scavenging system captures waste anesthetic gases and vents them away from personnel, reducing occupational exposure to isoflurane.
A mouse appears to be recovering from anesthesia but is hypothermic (rectal temperature 34°C). What is the most appropriate immediate action?
Answer: Place the mouse on a warm surface or under a heat lamp and monitor closely
Providing supplemental heat supports thermoregulation during anesthetic recovery, as hypothermia can delay recovery and cause cardiovascular depression.
Which method is considered the gold standard for confirming correct placement of an endotracheal tube in a research animal prior to a procedure?
Answer: Detecting CO2 on a capnograph (end-tidal CO2 monitoring)
Capnography (end-tidal CO2 detection) confirms that the tube is in the trachea by detecting exhaled CO2, making it the gold standard for tube placement verification.